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Uji Toksisitas Ekstrak, Fraksi N-Heksan, Etil Asetat, dan Metanol Kulit Labu Kuning (Cucurbita moschata) dengan Metode Brine Shrimp Lethality Test (BSLT)
Pengarang : Setiani, Slamet
Kata Kunci   :Artemia salina Leach, fraksi, kulit labu kuning, LC50, toksisitas

Kulit labu kuning (Cucurbita moschata) memiliki kandungan senyawa metabolit sekunder yang berpotensi memiliki aktivitas antikanker. Penelitian ini bertujuan untuk mengetahui nilai LC50 serta potensi ekstrak, fraksi n-heksan, etil asetat, dan metanol kulit labu kuning sebagai skrining awal aktivitas antikanker. Ekstraksi dilakukan dengan pelarut etanol, kemudian dilanjutkan dengan fraksinasi bertingkat menggunakan pelarut n-heksan, etil asetat, dan metanol. Selanjutnya dilakukan skrining fitokimia, penetapan kadar fenol menggunakan metode Folin-Ciocalteu, penetapan kadar β-karoten menggunakan spektrofotometri UV-Vis, serta uji toksisitas menggunakan metode Brine Shrimp Lethality Test (BSLT). Uji toksisitas dilakukan terhadap larva udang Artemia salina Leach dengan variasi konsentrasi 5, 10, 50, 100, dan 250 µg/mL, kemudian kematian larva diamati setelah 24 jam perlakuan. Nilai LC50 ditentukan menggunakan analisis probit. Penetapan kadar fenol menunjukkan bahwa fraksi metanol memiliki kadar fenol tertinggi sebesar 48,090 ± 1,576 mg GAE/g, sedangkan penetapan kadar β-karoten menunjukkan bahwa fraksi n-heksan memiliki kadar β-karoten tertinggi sebesar 245,736 ± 0,481 mg/g. Hasil uji BSLT menunjukkan bahwa nilai LC50 ekstrak sebesar 18,616 1,047 µg/mL, fraksi n-heksan sebesar 50,566 ± 0,886 µg/mL, fraksi etil asetat sebesar 8,970 ± 1,448 µg/mL, dan fraksi metanol sebesar 5,686 ± 0,662 µg/mL. Semua sampel memiliki nilai LC50 <1000 µg/mL, sehingga tergolong toksik terhadap larva Artemia salina Leach.
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